JUSTICE SAM

• Operated and monitored Detergent and Sulphonation plant via SCADA in the most efficient way to meet daily sales plan.
• Followed Standard Operating Procedures (SOPs) to ensure consistent production and product quality.
• Monitored process parameters such as temperature, pressure, flow rates, and chemical dosages to maintain optimal production conditions.
• Took samples for periodic quality checks to ensure product produced and raw materials meet the required specifications. Active Matter determination, Moisture, Acid Value, pH, Matter Insoluble in Alcohol, Bulk Density, and Color are some tests performed.
• Performed routine equipment inspection, cleaning, and maintenance to ensure proper functioning and minimize downtime.
• Took corrective action when required to prevent further disruption of service or damage to property.
• Investigated customer complaints relating to product quality and provided solutions accordingly.
• Troubleshot and resolved operational issues to minimize production interruptions.
• Inspected safety systems and determined necessary repairs or replacements.
• Participated in root cause analysis investigations following major incidents or accidents at the facility.
• Followed Safety Protocols and adhered to all relevant safety regulations to maintain a safe working environment.
• Assisted in training new operators on equipment handling and safety protocols.
• Implemented preventive maintenance procedures to enhance equipment reliability.
• Participated in continuous improvement initiatives to optimize plant processes.
• Prepared reports summarizing daily shift activity for management review.
• Coordinated ongoing maintenance and housekeeping of plant and equipment.

CTAB buffer DNA extraction protocol was used to extract the DNA from the Fasciola

(Flatworm).

• 200mg of parasite tissue was cut into tiny pieces and transferred into 1.5ml Eppendorf tube.
• Added 400µl of CTAB buffer to the parasite tissue.
• Incubated the CTAB/ parasite mixture for 15-20 minutes at 55℃ in a recirculating water bath.
• Spun the mixture at 5000 RPM for 24 minutes to spin down cell debris.
• Transferred the supernatant to clean 1.5 ml Eppendorf tubes.
• Added 200µl of chloroform: Iso Amyl Alcohol (24:1) to each tube and mixed the solution by inversion.
• Spun the tubes at 5000 RPM for 3 minutes.
• Transferred the upper aqueous phase only (contains the DNA) to a clean 2.0ml Eppendorf tube.
• Added 50µl of 7.5 M Ammonium Acetate to each tube.
• Added 500µl of ice-cold absolute ethanol.
• Tubes were placed for 1 hour at 20℃ after the addition of ethanol to precipitate the DNA. Tubes were spun at 1500 RPM for 5 minutes to precipitate DNA.
• Following precipitation,supernatant was pipetteed out leaving the DNA.

To wash the DNA,

• 500µl of ice-cold 70% ethanol was added, and tubes were slowly.
• DNA was spun into a pellet at 5000 RPM for 5 minutes after washing.
• Removed all the supernatant and allowed the DNA pellet to dry (approximately 15 minutes).
• DNA was resuspended in sterile DNase free water approximately 50-400µl H2O to remove any RNA in the preparation (10µl RNase in 10ml H2O).
• DNA was incubated at 65℃ for 20 minutes after resuspension to destroy any DNases that may be present and stored at 4℃.